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- Title
Characterization of a yam class IV chitinase produced by recombinant Pichia pastoris X-33.
- Authors
Akond, Muhammad Ali; Yusuke Matsuda; Takayuki Ishimaru; Ken Iwai; Akira Saito; Akio Kato; Shuhei Tanaka; Jun Kobayashi; Daizo Koga
- Abstract
The article presents a study that describes the characteristics of a yam class IV chitinase produced by recombinant Pichia pastoris X-33. It offers overview of the method of the study which purifies the chitinase through column chromatography after Endoglycosidase H treatment. It outlines the findings of the study which indicates that Pichia-produced chitinase showed strong lytic activity against Fusarium oxyporum and Phytophthora nicotianae.
- Subjects
CHITINASE; PICHIA pastoris; COLUMN chromatography; ENDOGLYCOSIDASES; LYSINS; FUSARIUM oxysporum; PHYTOPHTHORA nicotianae
- Publication
Bioscience, Biotechnology & Biochemistry, 2014, Vol 78, Issue 2, p222
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2014.885825