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- Title
Untitled.
- Authors
Chen, Hong; Zeng, Yong; Liu, Bi-Feng; Pang, Dai-Wen; Cheng, Jie-Ke
- Abstract
In microchip-based capillary electrophoresis, the resolution and separation efficiency of small peptides and amino acids can be noticeably improved by adding a low molecular weight (30,000) soluble polymer additive, polyvinypyrrolidone in the separation medium. Several separation conditions such as injection time and electrophoretic buffer have been investigated and optimized. Using an electro-stacking scheme, the resolution and separation efficiency of small peptides and amino acids can be enhanced significantly. Under the optimal conditions, the separation of fluorescein isothiocyanate Isomer I-labeled small peptides and amino acids was successfully achieved within 100 s.
- Publication
Analytical & Bioanalytical Chemistry, 2002, Vol 373, Issue 4/5, p314
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-002-1314-y