We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Third Generation ATP Sensor with Enzymatic Analyte Recycling.
- Authors
Yarman, Aysu; Schulz, Christopher; Sygmund, Cristoph; Ludwig, Roland; Gorton, Lo; Wollenberger, Ulla; Scheller, Frieder W.
- Abstract
For the first time the direct electron transfer of an enzyme - cellobiose dehydrogenase, CDH - has been coupled with the hexokinase catalyzed competition for glucose in a sensor for ATP. To enhance the signal output for ATP, pyruvate kinase was coimmobilized to recycle ADP by the phosphoenolpyruvate driven reaction. The new sensor overcomes the limit of 1 : 1 stoichiometry of the sequential or competitive conversion of ATP by effective enzymatic recycling of the analyte. The anodic oxidation of the glucose converting CDH proceeds at electrode potentials below 0 mV vs. Ag|AgCl thus potentially interfering substances like ascorbic acid or catecholamines do not influence the measuring signal. The combination of direct electron transfer of CDH with the enzymatic recycling results in an interference-free and oxygen-independent measurement of ATP in the lower µmolar concentration range with a lower limit of detection of 63.3 nM ( S/ N=3).
- Subjects
DETECTORS; ENZYMATIC analysis; WASTE recycling; ADENOSINE triphosphate; PYRUVATE kinase
- Publication
Electroanalysis, 2014, Vol 26, Issue 9, p2043
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201400231