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- Title
A Precise Chemical Strategy To Alter the Receptor Specificity of the Adeno-Associated Virus.
- Authors
Kelemen, Rachel E.; Mukherjee, Raja; Cao, Xiaofu; Erickson, Sarah B.; Zheng, Yunan; Chatterjee, Abhishek
- Abstract
The ability to target the adeno-associated virus (AAV) to specific types of cells, by altering the cell-surface receptor it binds, is desirable to generate safe and efficient therapeutic vectors. Chemical attachment of receptor-targeting agents onto the AAV capsid holds potential to alter its tropism, but is limited by the lack of site specificity of available conjugation strategies. The development of an AAV production platform is reported that enables incorporation of unnatural amino acids (UAAs) into specific sites on the virus capsid. Incorporation of an azido-UAA enabled site-specific attachment of a cyclic-RGD peptide onto the capsid, retargeting the virus to the αvβ3 integrin receptors, which are overexpressed in tumor vasculature. Retargeting ability was site-dependent, underscoring the importance of achieving site-selective capsid modification. This work provides a general chemical approach to introduce various receptor binding agents onto the AAV capsid with site selectivity to generate optimized vectors with engineered infectivity.
- Subjects
CELL receptors; ADENO-associated virus; CAPSIDS; BACTERIAL conjugation; AMINO acids; PEPTIDES; INTEGRINS
- Publication
Angewandte Chemie, 2016, Vol 128, Issue 36, p10803
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201604067