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- Title
Bioorthogonal Deprotection on the Dendritic Cell Surface for Chemical Control of Antigen Cross-Presentation.
- Authors
Pawlak, Joanna B.; Gential, Geoffroy P. P.; Ruckwardt, Tracy J.; Bremmers, Jessica S.; Meeuwenoord, Nico J.; Ossendorp, Ferry A.; Overkleeft, Herman S.; Filippov, Dmitri V.; van Kasteren, Sander I.
- Abstract
The activation of CD8+ T-cells requires the uptake of exogenous polypeptide antigens and proteolytic processing of these antigens to octamer or nonamer peptides, which are loaded on MHC-I complexes and presented to the T-cell. By using an azide as a bioorthogonal protecting group rather than as a ligation handle, masked antigens were generated-- antigens that are not recognized by their cognate T-cell unless they are deprotected on the cell using a Staudinger reduction.
- Subjects
T cells; POLYPEPTIDES; ANTIGENS; PROTEOLYTIC enzymes; PEPTIDES; CELL membranes; BIOCHEMISTRY
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 19, p5720
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201500301