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- Title
Preparation and characterization of bacterial cellulose by kombucha using corncob.
- Authors
Liu, Zhanna; Sun, Qihang; Wang, Yingying; Liu, Jie; Zhu, Ping
- Abstract
Bacterial cellulose (BC) is widely used in various fields due to its excellent physicochemical properties. However, the main drawback is its high production cost. The present research exploited the preparation of BC by combining the hydrothermal process using acetic acid and enzyme treatment for the hydrolysis of corncob. The BC production was further improved by optimizing the key factors. The concentration of reducing sugar was 22.7 g/L after thermohydrolysis at 180 °C for 80 min and enzyme hydrolysis in 6 U/mL cellulase buffer solution for 24 h. It supported about 1.36 g/L as the sole culture medium, which was 5.6 times more than corncob hydrolysate without detoxification (0.25 g/L) and more than Hestrin-Schramm (HS) standard medium (1.12 g/L). The BC products were characterized by FT-IR spectroscopy, XRD, TG, and SEM analysis. Compared to the BC produced by HS medium, the morphology of BC produced by detoxification corncob hydrolysate showed a lower value of the coefficient of variation (37.59%) and higher mean diameter (55.23 nm). XRD data showed higher crystallinity (77.50%) and TG analysis showed high thermal stability. The current study demonstrated that corncob is a promising carbon source for the sustainable production of BC.
- Subjects
SUSTAINABILITY; CORNCOBS; X-ray diffraction; BUFFER solutions; INDUSTRIAL costs
- Publication
Cellulose, 2024, Vol 31, Issue 11, p6625
- ISSN
0969-0239
- Publication type
Article
- DOI
10.1007/s10570-024-06018-0