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- Title
Cep131-Cep162 and Cby-Fam92 complexes cooperatively maintain Cep290 at the basal body and contribute to ciliogenesis initiation.
- Authors
Wu, Zhimao; Chen, Huicheng; Zhang, Yingying; Wang, Yaru; Wang, Qiaoling; Augière, Céline; Hou, Yanan; Fu, Yuejun; Peng, Ying; Durand, Bénédicte; Wei, Qing
- Abstract
Cilia play critical roles in cell signal transduction and organ development. Defects in cilia function result in a variety of genetic disorders. Cep290 is an evolutionarily conserved ciliopathy protein that bridges the ciliary membrane and axoneme at the basal body (BB) and plays critical roles in the initiation of ciliogenesis and TZ assembly. How Cep290 is maintained at BB and whether axonemal and ciliary membrane localized cues converge to determine the localization of Cep290 remain unknown. Here, we report that the Cep131-Cep162 module near the axoneme and the Cby-Fam92 module close to the membrane synergistically control the BB localization of Cep290 and the subsequent initiation of ciliogenesis in Drosophila. Concurrent deletion of any protein of the Cep131-Cep162 module and of the Cby-Fam92 module leads to a complete loss of Cep290 from BB and blocks ciliogenesis at its initiation stage. Our results reveal that the first step of ciliogenesis strictly depends on cooperative and retroactive interactions between Cep131-Cep162, Cby-Fam92 and Cep290, which may contribute to the complex pathogenesis of Cep290-related ciliopathies. Cilia play critical roles in cell signal transduction and organ development. This study shows that the Cep131-Cep162 module near the axoneme and the Cby-Fam92 module near the membrane work together to regulate the localization of the highly conserved ciliopathy protein Cep290 at the basal body to initiate ciliogenesis.
- Subjects
MORPHOGENESIS; CELL communication; CELLULAR signal transduction; GENETIC disorders; CILIA &; ciliary motion; ORGANS (Musical instruments)
- Publication
PLoS Biology, 2024, Vol 22, Issue 3, p1
- ISSN
1544-9173
- Publication type
Article
- DOI
10.1371/journal.pbio.3002330