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- Title
Cytoplasmic basophilia in the nerve cells of the cerebral cortex.
- Authors
Pakkenberg, H.; Vraa-Jensen, J.
- Abstract
Various inhibitors of glycolysis (0.1 mol monoiodoacetic acid, fluoracetic acid, sodium fluoride, sodium bisulphite, and a mixture of phenobarbital and monoiodoacetic acid) were used on tissue from the cerebral cortex and cerebellum of guinea pigs. Monoiodoacetic acid and fluoracetic acid were found to produce such pronounced artefacts in the cells that they could not be used as inhibitors. Sodium fluoride and sodium sulphite also produce artefacts, in the form of 'holes' (loss of substance) in the cytoplasm, though to a lesser degree than the first two inhibitors mentioned. A mixture of phenobarbital and monoiodoacetic acid (p 7) can be used, but offers no advantages over ordinary fixation 2 hrs after death, i.e. the lapse of time the other tissue samples had lain in inhibitor. With gallocyanin staining and determination of the extinction in the cytoplasm of the large nerve cells, measurements up to 4 days post mortem show a demonstrable loss of RNA on the 2nd day. As there are cell volume changes in the form of an initial shrinkage followed by an increase in the volume, the volume must be allowed for in relation to the RNA concentration in order to evaluate the changes in the RNA content of the cytoplasm of the cells.
- Publication
Acta Neuropathologica, 1964, Vol 3, Issue 3, p211
- ISSN
0001-6322
- Publication type
Article
- DOI
10.1007/BF00684396