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- Title
Transformation of Nasturtium officinale, Barbarea verna and Arabis caucasica for hairy roots and glucosinolate-myrosinase system production.
- Authors
Wielanek, Marzena; Królicka, Aleksandra; Bergier, Katarzyna; Gajewska, Ewa; Skłodowska, Maria
- Abstract
Hairy roots of Nasturtium officinale, Barbarea verna and Arabis caucasica with active glucosinolate-myrosinase system were obtained after transformation with Agrobacterium rhizogenes. Hairy roots of N. officinale produced phenylalanine-derived gluconasturtiin and glucotropaeolin (max. 24 and 7 mg g−1 DW). B. verna and A. caucasica hairy roots produced gluconasturtiin (max. 41 mg g−1 DW) and methionine-derived glucoiberverin (max. 32 mg g−1 DW), respectively. Treatment of the roots with amino acid precursors of glucosinolate or/and cysteine biosynthesis increased levels of glucosinolate production, combinations of phenylalanine with cysteine (for gluconasturtiin and glucotropaeolin) and methionine with o-acetylserine (for glucoiberverin) were the most effective.
- Subjects
WATERCRESS; EARLY yellowrocket; ARABIS; ROOT hairs (Botany); AMINO acids; GLUCOSINOLATES; BIOSYNTHESIS
- Publication
Biotechnology Letters, 2009, Vol 31, Issue 6, p917
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-009-9953-0