We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
锌离子响应转录激活因子 ZafA 对球孢白僵菌锌 离子利用及生防潜能的影响.
- Authors
李芳; 张珏锋; 钟海英; 陈建明
- Abstract
Beauveria bassiana, as a hyphomycete pathogenic fungus, is widely used in the control of agricultural and forestry insect pests. However, the changeable environment in the field limits the effectiveness of the fungal agent. In addition, the environment in vivo of the host also affects the proliferation and infection rate of the fungus. In order to study the balance ability of the fungus to environmental pH and trace elements, the relationship between zinc-responsive transcriptional activator ZafA and B. bassiana growth and reproduction, stress resistance, virulence and zinc utilization was preliminarily explored. The results showed that deletion of zafA resulted in reduction of the ability of fungal growth and reproduction and spore germination, and the sensitivity to oxidation, hyperosmotic, cell wall interfering agents and UV stress increased, bringing about the decline of virulence and expression level of related trait genes. The knockout strains could not grow without zinc ion. The expression levels of zafA and zinc ion transporter coding genes zrf1–8 were affected by pH and zinc ion concentration. Thus, zafA not only restricts the utilization of zinc ion of B. bassiana, but also affects the stress resistance and virulence of the fungus. This study provides a new evidence for improving environmental adaptability and virulence of the fungi used for biocontrol.
- Subjects
ZINC transporters; ZINC ions; BEAUVERIA bassiana; FUNGAL virulence; FUNGAL growth; OSMOREGULATION
- Publication
Mycosystema, 2022, Vol 41, Issue 4, p570
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.210337