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- Title
NANOG expression in parthenogenetic porcine blastocysts is required for intact lineage specification and pluripotency.
- Authors
Mingyun Lee; Jong-Nam Oh; Gyung Cheol Choe; Kwang-Hwan Choi; Dong-Kyung Lee; Seung-Hun Kim; Jinsol Jeong; Yelim Ahn; Chang-Kyu Lee
- Abstract
Objective: Nanog homeobox (NANOG) is a core transcription factor that contributes to pluripotency along with octamer binding transcription factor-4 (OCT4) and sex determining region-Y box-2 (SOX2). It is an epiblast lineage marker in mammalian pre-implantation embryos and exhibits a species-specific expression pattern. Therefore, it is important to understand the lineage of NANOG, the trophectoderm, and the primitive endoderm in the pig embryo. Methods: A loss- and gain-of-function analysis was done to determine the role of NANOG in lineage specification in parthenogenetic porcine blastocysts. We analyzed the relationship between NANOG and pluripotent core transcription factors and other lineage makers. Results: In NANOG-null late blastocysts, OCT4-, SOX2-, and SOX17-positive cells were decreased, whereas GATA binding protein 6 (GATA6)-positive cells were increased. Quantitative real-time polymerase chain reaction revealed that the expression of SOX2 was decreased in NANOG-null blastocysts, whereas that of primitive endoderm makers, except SOX17, was increased. In NANOG-overexpressing blastocysts, caudal type homeobox 2 (CDX2-), SOX17-, and GATA6-positive cells were decreased. The results indicated that the expression of primitive endoderm markers and trophectoderm-related genes was decreased. Conclusion: Taken together, the results demonstrate that NANOG is involved in the epiblast and primitive endoderm differentiation and is essential for maintaining pluripotency within the epiblast.
- Subjects
BLASTOCYST; MAMMALIAN embryos; GATA proteins; ENDODERM; POLYMERASE chain reaction; HOMEOBOX genes
- Publication
Animal Bioscience, 2023, Vol 36, Issue 12, p1905
- ISSN
2765-0189
- Publication type
Article
- DOI
10.5713/ab.23.0210