We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
HPLC determination of quercetin using relative molar sensitivity to methylparaben as a single reference.
- Authors
Yuzo Nishizaki; Kyoko Ishizuki; Naoko Masumoto; Atsuko Tada; Naoki Sugimoto; Kyoko Sato
- Abstract
A simple and reliable high-performance liquid chromatography (HPLC) method for the determination of quercetin (QR) was developed. The method requires no QR reference; instead,it uses relative molar sensitivity (RMS) to a single reference, methylparaben (MPB). The RMS of QR/MPB was determined using an offline combination of -quantitative NMR H-qNMR) and HPLC/UV or HPLC/photodiode array (PDA). In this study,the RMS represents the sensitivity ratio of QR to MPB per mole under defined HPLC conditions. The QR in two natural food additives was quantified using three different techniques such as iH-qNMR,UV-Vis spectrophotometry, and our single-reference HPLC method. Based on certain amounts of QR in two food additive products,the similar results using -qNMR (mass fraction: 96.9% and 96.1%) and single-reference HPLC (95.8% and 95.0%) were exhibited. On the other hand,the results using UV-Vis spectrophotometry (98.5% and 99.2%) without separation of QR and others (kaempferol and isorhamnetin impurities) showed slightly higher amounts than other techniques. In these techniques, the single-reference HPLC is advanced for the simple and accurate quantification of QR in food additive products without native standard.
- Subjects
LIQUID chromatography; HIGH performance liquid chromatography; QUERCETIN; PHOTODIODES; FOOD additives
- Publication
Japanese Journal of Food Chemistry & Safety, 2020, Vol 27, Issue 2, p42
- ISSN
1341-2094
- Publication type
Article