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- Title
MicroRNA-495 对人脐静脉内皮细胞ICAM-1 表达的影响.
- Authors
刘丹; 刘美丽; 刘艳霞; 田孝祥; 齐艳萍
- Abstract
Objective: To investigate the effect of rnicroRNA-495 (miR495) on the expression of intercellular adhesion inoleciile-1 (ICAM-1) in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs cells cultured in vitro were plated into 6-weIl plate, when cells were grown to 80 %. miR495 mimics. niiR.495 inhibitor or negative control (NC), inhibitor NC was transfected into HUVECs. At different time points after transaction (12 h, 24 h and 48 h).HUVECs were collected for RNA and protein extraction. The mRNA expressions of miR495 and ICAM-1 were examined using quantitative real time RT-PCR. ICAM-1 protein expression was examined using western blotting. Results: (1) Compared with NC group. miR495 expression was significantly higher in miR495 mimics group; Compared with inhibitor NC group. miR495 expression was significantly decreased in iniR495 inhibitor group. (2) Compared with NC. rniR495 significantly inhibited the niRNA and protein expressions of ICAM-1 in HUVECs; Compared with inhibitor NC. miR495 inhibitor significantly increased the mRNA and protein expressions of ICAM-1 in HUVECs. Conclusions: MiR495 could affect ICAM-1 expression in HUVECs.
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 12, p2253
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.12.011