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- Title
LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY METHODS FOR THE DETERMINATION OF ANDARINE AND OSTARINE IN RAT SERUM AFTER PROTEIN PRECIPITATION.
- Authors
MIKLOS, AMALIA; TERO-VESCAN, AMELIA; FARCZÁDI, LENÁRD; IMRE, SILVIA; VARI, CAMIL-EUGEN; MUNTEAN, DANIELA-LUCIA
- Abstract
Quantitative determination of andarine and ostarine in rat serum was achieved using two selective liquid chromatographymass spectrometry methods (LC-MS). After extracting the analytes and internal standards (ostarine and andarine) from the serum samples through a high throughput extraction method (protein precipitation), the samples were analysed by reverse phase liquid chromatography using as mobile phase a mixture of methanol and 10 mM ammonium formate solution (75:25, v/v). The analytes were detected following specific transitions in MS/MS mode of the QTOF mass spectrometer, after preionization by a negative electrospray ionization source (ESI-). Both methods were validated for a concentration range between 50 - 10000 ng/mL according to the current official guidelines with regards to carry-over, selectivity, linearity, within- and between-run accuracy, precision and can be applied to accurately measure andarine and ostarine levels in rat serum samples in order to design a pharmacokinetic study or for doping control purposes.
- Subjects
LIQUID chromatography-mass spectrometry; BLOOD proteins; REVERSE phase liquid chromatography; MOBILE phase (Chromatography); RATS
- Publication
Farmacia, 2019, Vol 67, Issue 5, p772
- ISSN
0014-8237
- Publication type
Article
- DOI
10.31925/farmacia.2019.5.4