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- Title
Next‐generation sequencing using tissue specimen collected with a 1.1 mm‐diameter cryoprobe in patients with lung cancer.
- Authors
Kim, Mi‐Hyun; Kim, Soo Han; Lee, Geewon; Mok, Jeongha; Lee, Min Ki; Song, Ju Sun; Eom, Jung Seop
- Abstract
Background and Objective: Next‐generation sequencing (NGS) analysis is considered standard for lung cancer diagnosis in clinical practice. Little is known about the feasibility of NGS using tumour tissue sampled with a 1.1 mm‐diameter cryoprobe. We aimed to investigate the suitability of specimens obtained by transbronchial cryobiopsy (TBC) using a 1.1 mm‐diameter cryoprobe for NGS analysis. Methods: Patients with lung cancer who underwent TBC using a 1.1 mm‐diameter cryoprobe for NGS testing between October 2020 and April 2023 were enrolled. A 4.0‐ or 3.0 mm‐diameter bronchoscope with radial probe endobronchial ultrasound and virtual bronchoscopic navigation was used to detect peripheral lung lesions. All procedures were performed under fluoroscopic guidance. Data were analysed retrospectively. Results: A total of 56 patients underwent TBC using a 1.1 mm cryoprobe for NGS testing, during the study period. Most patients (98%) were in the advanced stage of lung cancer (recurrent or inoperable disease of stages III or IV). The diagnostic yield of NGS for DNA and RNA sequencing was 95% each (53 of 56). Moderate bleeding was noted in three patients (5%) and none of the study patients developed life‐threatening complications, such as pneumothorax or lung infection. Conclusion: TBC using a 1.1 mm‐diameter cryoprobe is a useful and safe tool for NGS analysis, for both DNA and RNA sequencing.
- Subjects
NUCLEOTIDE sequencing; LUNG cancer; BRONCHOSCOPES; CANCER patients; RNA sequencing; LUNG diseases
- Publication
Respirology, 2024, Vol 29, Issue 4, p333
- ISSN
1323-7799
- Publication type
Article
- DOI
10.1111/resp.14680