We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA.
- Authors
Ding, Yingying; He, Ting; Feng, Jiaojiao; Gao, Caixia; Wang, Lili; Wang, Jinhong; Li, Xiangyu; Cao, Mingmei; Peng, Heng; Zhao, Chunyan; Pan, Wei; Chen, Xuguang; Qian, Baohua; Wu, Guorong
- Abstract
Human enterovirus 71 (EV71) has become the major pathogen of hand, foot, and mouth disease (HFMD) worldwide, while the anti-EV71 antibody responses other than neutralizing epitopes have not been characterized. In this study, EV71 capsid proteins VP1, VP3, VP0 and various VP1 antigens were constructed to analyze anti-EV71 response in severe HFMD cases, non-HFMD outpatient children and normal adults using a novel evolved immunoglobulin-binding molecule (NEIBM)-based ELISA. The high prevalence of antibody responses against all three capsid proteins was demonstrated, and anti-EV71 VP1 showed the main antibody response. Anti-EV71 VP1 antibody response was found to predominantly target to epitopes based on the common enterovirus cross-reactive sequence. Moreover, inhibition pattern against anti-EV71 VP1 reactions in three groups was obviously different. Taken together, these results firstly characterized the anti-EV71 antibody responses which are predominantly against VP1 epitopes based on common enterovirus cross-reactive sequence. This finding could be helpful for the better understanding of anti-EV71 humoral immunity and useful for seroepidemiological surveillance.
- Subjects
ANTIBODY formation; ENTEROVIRUSES; HAND, foot &; mouth disease; ENZYME-linked immunosorbent assay; CAPSIDS; PROTEIN expression
- Publication
Scientific Reports, 2015, p10636
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep10636