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- Title
Thirteen Ovary-Enriched Genes Are Individually Not Essential for Female Fertility in Mice.
- Authors
Pham, Anh Hoang; Emori, Chihiro; Ishikawa-Yamauchi, Yu; Tokuhiro, Keizo; Kamoshita, Maki; Fujihara, Yoshitaka; Ikawa, Masahito
- Abstract
Infertility is considered a global health issue as it currently affects one in every six couples, with female factors reckoned to contribute to partly or solely 50% of all infertility cases. Over a thousand genes are predicted to be highly expressed in the female reproductive system and around 150 genes in the ovary. However, some of their functions in fertility remain to be elucidated. In this study, 13 ovary and/or oocyte-enriched genes (Ccdc58, D930020B18Rik, Elobl, Fbxw15, Oas1h, Nlrp2, Pramel34, Pramel47, Pkd1l2, Sting1, Tspan4, Tubal3, Zar1l) were individually knocked out by the CRISPR/Cas9 system. Mating tests showed that these 13 mutant mouse lines were capable of producing offspring. In addition, we observed the histology section of ovaries and performed in vitro fertilization in five mutant mouse lines. We found no significant anomalies in terms of ovarian development and fertilization ability. In this study, 13 different mutant mouse lines generated by CRISPR/Cas9 genome editing technology revealed that these 13 genes are individually not essential for female fertility in mice.
- Subjects
FERTILITY; GENITALIA; FERTILIZATION in vitro; GENOME editing; GENES; OVARIAN follicle; INFERTILITY; MALE infertility
- Publication
Cells (2073-4409), 2024, Vol 13, Issue 10, p802
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells13100802