We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Heparan Sulfate Modulation Affects Breast Cancer Cell Adhesion and Transmigration across In Vitro Blood–Brain Barrier.
- Authors
Li, Yunfei; Shteyman, David B.; Hachem, Zeina; Ulay, Afaf A.; Fan, Jie; Fu, Bingmei M.
- Abstract
The disruption of endothelial heparan sulfate (HS) is an early event in tumor cell metastasis across vascular barriers, and the reinforcement of endothelial HS reduces tumor cell adhesion to endothelium. Our recent study showed that while vascular endothelial growth factor (VEGF) greatly reduces HS at an in vitro blood–brain barrier (BBB) formed by human cerebral microvascular endothelial cells (hCMECs), it significantly enhances HS on a breast cancer cell, MDA-MB-231 (MB231). Here, we tested that this differential effect of VEGF on the HS favors MB231 adhesion and transmigration. We also tested if agents that enhance endothelial HS may affect the HS of MB231 and reduce its adhesion and transmigration. To test these hypotheses, we generated an in vitro BBB by culturing hCMECs on either a glass-bottom dish or a Transwell filter. We first quantified the HS of the BBB and MB231 after treatment with VEGF and endothelial HS-enhancing agents and then quantified the adhesion and transmigration of MB231 across the BBB after pretreatment with these agents. Our results demonstrated that the reduced/enhanced BBB HS and enhanced/reduced MB231 HS increase/decrease MB231 adhesion to and transmigration across the BBB. Our findings suggest a therapeutic intervention by targeting the HS-mediated breast cancer brain metastasis.
- Subjects
BLOOD-brain barrier; CELL migration; HEPARAN sulfate; VASCULAR endothelial growth factors; CANCER cells; BREAST cancer; CELL adhesion
- Publication
Cells (2073-4409), 2024, Vol 13, Issue 2, p190
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells13020190