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- Title
Plant MIR167e-5p Inhibits Enterocyte Proliferation by Targeting β-Catenin.
- Authors
Li, Meng; Chen, Ting; He, Jia-Jian; Wu, Jia-Han; Luo, Jun-Yi; Ye, Rui-Song; Xie, Mei-Ying; Zhang, Hao-Jie; Zeng, Bin; Liu, Jie; Xi, Qian-Yun; Jiang, Qing-Yan; Sun, Jia-Jie; Zhang, Yong-Liang
- Abstract
MicroRNAs (miRNAs) are important negative regulators of genes involved in physiological and pathological processes in plants and animals. It is worth exploring whether plant miRNAs play a cross-kingdom regulatory role in animals. Herein, we found that plant MIR167e-5p regulates the proliferation of enterocytes in vitro. A porcine jejunum epithelial cell line (IPEC-J2) and a human colon carcinoma cell line (Caco-2) were treated with 0, 10, 20, and 40 pmol of synthetic 2′-O-methylated plant MIR167e-5p, followed by a treatment with 20 pmol of MIR167e-5p for 0, 24, 48, and 72 h. The cells were counted, and IPEC-J2 cell viability was determined by the MTT and EdU assays at different time points. The results showed that MIR167e-5p significantly inhibited the proliferation of enterocytes in a dose- and time-dependent manner. Bioinformatics prediction and a luciferase reporter assay indicated that MIR167e-5p targets β-catenin. In IPEC-J2 and Caco-2 cells, MIR167e-5p suppressed proliferation by downregulating β-catenin mRNA and protein levels. MIR167e-5p relieved this inhibition. Similar results were achieved for the β-catenin downstream target gene c-Myc and the proliferation-associated gene PCNA. This research demonstrates that plant MIR167e-5p can inhibit enterocyte proliferation by targeting the β-catenin pathway. More importantly, plant miRNAs may be a new class of bioactive molecules for epigenetic regulation in humans and animals.
- Subjects
REGULATOR genes; COLON (Anatomy); EPITHELIAL cells; CELL survival; PLANT capacity; CELL lines
- Publication
Cells (2073-4409), 2019, Vol 8, Issue 11, p1385
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells8111385