We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Immobilized Cell Physiology Imaging and Stabilization of Enzyme Cascade Reaction Using Recombinant Cells Escherichia coli Entrapped in Polyelectrolyte Complex Beads by Jet Break-Up Encapsulator.
- Authors
Bučko, Marek; Gemeiner, Peter; Krajčovič, Tomáš; Hakarová, Marietta; Chorvát, Dušan; Chorvátová, Alžbeta Marček; Lacík, Igor; Rudroff, Florian; Mihovilovič, Marko D.
- Abstract
A novel, high performance, and scalable immobilization protocol using a laminar jet break-up technique was developed for the production of polyelectrolyte complex beads with entrapped viable Escherichia coli cells expressing an enzyme cascade of alcohol dehydrogenase, enoate reductase, and cyclohexanone monooxygenase. A significant improvement of operational stability was achieved by cell immobilization, which was manifested as an almost two-fold higher summative product yield of 63% after five cascade reaction cycles as compared to the yield using free cells of 36% after the maximum achievable number of three cycles. Correspondingly, increased metabolic activity was observed by multimodal optical imaging in entrapped cells, which was in contrast to a complete suppression of cell metabolism in free cells after five reaction cycles. Additionally, a high density of cells entrapped in beads had a negligible effect on bead permeability for low molecular weight substrates and products of cascade reaction.
- Subjects
IMMOBILIZED cells; IMAGE stabilization; CELL imaging; ESCHERICHIA coli; ALCOHOL dehydrogenase; CELL physiology
- Publication
Catalysts (2073-4344), 2020, Vol 10, Issue 11, p1288
- ISSN
2073-4344
- Publication type
Article
- DOI
10.3390/catal10111288