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- Title
ISOLATION AND CHARACTERIZATION OF WILD STRAINS OF AGROBACTERIUM FROM CROWN GALL INFECTED PLANTS.
- Authors
Khan, Aliullah; Yasmin, Aneela; Rind, Rafique; Abro, Manzoor; Abro, Shahid H.; Abro, Rani
- Abstract
Agrobacterium tumefaciens is a plant pathogen that has immense importance in plant genetic engineering. Its pathogenic strains have a large tumor inducing plasmid that can transfer and integrate transfer DNA segment in the plant genome andhelp in generation of transgenics. The highly virulent bacteria can transform plant cells more efficiently as compared to non-virulent strains. Keeping in view this fact twenty-five wild strains of Agrobacterium were isolated from infected plants of Mango, Sitaashok, Chikoo, Neem, Eucalyptus and Gulmohar growing in the Horticulture Garden, Sindh Agriculture University, Tandojam. Fifteen isolates out of twenty-five were designated as A.tumefaciens on the basis of morphological, biochemical and phyto-pathological tests. Among these fifteen isolates, three strains were found to be nonpathogenic whereas the remaining revealed tumor forming ability on carrot discs in an in vitro disease assay. Isolate AtMi003 of A. tumefacienswas the most virulent strain on the basis of tumor quantity generated and analyzed through ANOVA and LSD at p= 0.05. It showed 80% transformation efficiency and 23.67 mg tumor weight on carrot discs.
- Subjects
BACTERIAL diseases of plants; AGROBACTERIUM; PLANT genetic engineering
- Publication
Pakistan Journal of Phytopathology, 2016, Vol 28, Issue 2, p121
- ISSN
1019-763X
- Publication type
Article