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- Title
Homologous Recombination Is Required for Genome Stability in the Absence of DOG-i in Caenorhabditis elegans.
- Authors
Youds, Jillian L.; O'Neil, Nigel J.; Rose, Ann M.
- Abstract
In C. elegans, DOC-1 prevents deletions that initiate in polyG/polyC tracts (G/C tracts), most likely by unwinding secondary structures that can form in G/C tracts during lagging-strand DNA synthesis. We have used the dog-1 mutant to assay the in vivo contribution of various repair genes to the maintenance of G/C tracts. Here we show that DOC-1 and the BLM ortholog, HIM-6, act synergistically during replication; simultaneous loss of function of both genes results in replicative stress and an increase in the formation of small deletions that initiate in G/C tracts. Similarly, we demonstrate that the C. elegans orthologs of the homologous recombination repair genes BARD1, RAD51, and XPF and the trans-lesion synthesis polymerases pol-η and polκ contribute to the prevention of deletions in dog-1 mutants. Finally, we provide evidence that the small deletions generated in the dog-1 background are not formed through homologous recombination, nucleotide excision repair, or nonhomologous end-joining mechanisms, but appear to result from a mutagenic repair mechanism acting at G/C tracts. Our data support the hypothesis that absence of DOG-1 leads to replication fork stalling that can be repaired by deletion-free or deletion-prone mechanisms.
- Subjects
CAENORHABDITIS elegans; GENOMES; GENOMICS; DNA synthesis; CAENORHABDITIS; GENETIC mutation; MUTAGENESIS
- Publication
Genetics, 2006, Vol 173, Issue 2, p697
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.106.056879