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- Title
The Requirements for COMPASS and Paf 1 in Transcriptional Silencing and Methylation of Histone H3 in Saccharomyces cerevisiae.
- Authors
Mueller, John E.; Canze, Megan; Bryk, Mary
- Abstract
The SeU-containing complex, COMPASS, methylates histone H3 On lysine 4 (K4) in Saccharoinyces cerevisiae. Despite the preferential association of K4.trimethylated H3 with regions of the genome that are transcribed by RNA polyinerase II, transcriptional silencing is one of the few cases in S. cerevisiae where histone-methylation defects have a clear effect on gene expression. To better understand the role of COMPASS in transcriptional silencing, we have determined which members of COMPASS are required for silencing at the ribosomal DNA locus (rDNA), a telomere, and the silent mating loci (HAl) using Northern analyses. Our findings indicate that most members of COMPASS are required for silencing at the rDNA and telomere, while none are required for silencing of endogenous genes at the HMloci. To complement gene- expression analysis, quantitative Western blot experiments were performed to determine the members of COMPASS that are required for methylation of histone H3. While most are required for trimethylation, cells lacking certain COMPASS proteins maintain reduced levels of K4 mono- and dimethylated H3, suggesting that some COMPASS members have redundant function. Finally, we show Pafl is required for silencing and K4-methylated H3 at the rDNA, suggesting a possible direct role for K4-methylated H3 in gene silencing.
- Subjects
METHYLATION; HISTONES; SACCHAROMYCES cerevisiae; GENETIC regulation; BASIC proteins; GENOMES
- Publication
Genetics, 2006, Vol 173, Issue 2, p557
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.106.055400