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- Title
Changes in the microbiota in different intestinal segments of mice with sepsis.
- Authors
Yahui Peng; Jieling Wei; Xiaonan Jia; Feiyu Luan; Mingyin Man; Xiaohui Ma; Yinghao Luo; Yue Li; Nana Li; Qian Wang; Xibo Wang; Yang Zhou; Yuanyuan Ji; Wenjing Mu; Jun Wang; Chunying Wang; Qianqian Zhang; Kaijiang Yu; Mingyan Zhao; Changsong Wang
- Abstract
Introduction: The small intestine, as the main digestion and absorption site of the gastrointestinal tract, is often overlooked in studies, and the overall microbiota does not reflect the makeup of the microbiota in different segments of the intestine. Therefore, we aimed to exclude the influence of routine ICU treatment measures on sepsis patients and observed changes in the diversity and abundance of gut microbiota in different intestinal segments of septic mice. Methods: The mice were randomly divided into the CLP6h group and the sham group. The contents of the colon and small intestine of the experimental group and the control group were collected after 6 h. Results: After CLP, the number and structure of the gut microbiota in the colon changed most obviously, among which Bacteroidetes had the most significant changes. Akkermansia, D.Firmicutes_bacterium_M10_2, Blautia, Bifidobacterium, Lactobacillus, Candidatus_Arthromitus, and Muribaculaceae were changed in the colon. Lactobacillus, Bifidobacterium, Akkermansia, Blautia, Candidatus_Arthromitus, and Lachnospiraceae_NK4A136_group were changed in the small intestine. Discussion: Our experiment found that there were different numbers of unique and common gut microbiota in the small intestine and colon after sepsis, and the gut microbiota of the colon changed more drastically after sepsis than the small intestine. Thus, we should focus on protective gut microbiota and mucindegrading microbes. We hope that these results will provide help for sepsis treatment in the future.
- Subjects
SEPSIS; GUT microbiome; SMALL intestine; GASTROINTESTINAL system; MICE; BIFIDOBACTERIUM
- Publication
Frontiers in Cellular & Infection Microbiology, 2023, Vol 12, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2022.954347