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- Title
Synthesis of low molecular weight thiols in response to Cd exposure in Thlaspi caerulescens.
- Authors
Hernández-Allica, J.; C.Garbisu; Becerril, J. M.; Barrutia, O.; Garc�ED;a-Plazaola, J. I.; Zhao, F. J.; McGrath, S. P.
- Abstract
In this study, we investigated the accumulation of phytochelatins (PCs) and other low molecular weight (LMW) thiols in response to Cd exposure in two contrasting ecotypes differing in Cd accumulation. Using a root elongation test, we found that the highly accumulating ecotype Ganges was more tolerant to Cd than the low Cd-accumulation ecotype Prayon.l-buthionine-(S,R)-sulphoximine (BSO), a potent inhibitor of the γ-glutamylcysteine synthetase ( γ-ECS) (an enzyme involved in the PC biosynthetic pathway), increased the Cd sensitivity of Prayon, but had no effect on Ganges. Although PC accumulation increased in response to Cd exposure, no significant differences were observed between the two ecotypes. Cd exposure induced a dose-dependent accumulation of both Cys and a still unidentified LMW thiol in roots of both ecotypes. Root accumulation of Cys and this thiol was higher in Ganges than in Prayon; the ecotypic differences were more pronounced when the plants were treated with BSO. These findings suggest that PCs do not contribute to the Cd hypertolerance displayed by the Ganges ecotype of Thlaspi caerulescens, whereas Cys and other LMW thiols might be involved.
- Subjects
THIOLS; THLASPI; ENZYMES; PEPTIDES; PROTEINS; PLANT roots; CRYOSCOPY; PHYSICAL &; theoretical chemistry; ORGANOSULFUR compounds; CAPTOPRIL
- Publication
Plant, Cell & Environment, 2006, Vol 29, Issue 7, p1422
- ISSN
0140-7791
- Publication type
Article
- DOI
10.1111/j.1365-3040.2006.01525.x