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- Title
Osmotic properties of Limulusseawaters and organ cultures: An unrecognized issue.
- Authors
CORRINNE C.M. LIM-KESSLER; AMANDA R. BOLBECKER; JIA LI; GERALD S. WASSERMAN
- Abstract
Excised eyes of the horseshoe crab (Limulus polyphemus) have long provided robust preparations for fundamental studies of the visual neuroscience of photoreceptors. Such preparations are particularly useful for immersion in perfusion chambers whose perfusate can be varied as needed. Little attention has been paid in the past to the osmotic properties of such perfusates because they have generally been virtually isotonic with natural seawater. We here report that (1) some recent Limulusstudies have used organ culture perfusates that are severely hypotonic and (2) that that fact has not been adequately disclosed.
- Subjects
EYE; LIMULUS polyphemus; NEUROSCIENCES; PHOTORECEPTORS; SEAWATER
- Publication
Visual Neuroscience, 2008, Vol 25, Issue 1, p103
- ISSN
0952-5238
- Publication type
Article
- DOI
10.1017/S0952523808080115