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- Title
A Beginner's Guide to Cell Culture: Practical Advice for Preventing Needless Problems.
- Authors
Weiskirchen, Sabine; Schröder, Sarah K.; Buhl, Eva Miriam; Weiskirchen, Ralf
- Abstract
The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation research. However, despite their important role, cell lines are frequently misidentified or contaminated by other cells, bacteria, fungi, yeast, viruses, or chemicals. In addition, handling and manipulating of cells is associated with specific biological and chemical hazards requiring special safeguards such as biosafety cabinets, enclosed containers, and other specialized protective equipment to minimize the risk of exposure to hazardous materials and to guarantee aseptic work conditions. This review provides a brief introduction about the most common problems encountered in cell culture laboratories and some guidelines on preventing or tackling respective problems.
- Subjects
ARTIFICIAL cells; HAZARDOUS substance exposure; MOLECULAR biology; CYTOLOGY; CELL culture; SAFETY appliances
- Publication
Cells (2073-4409), 2023, Vol 12, Issue 5, p682
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells12050682