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- Title
Application of Molecular Marker Assayed Paternity in a White Clover Breeding Program.
- Authors
George, R. M.; Woodfield, D. R.; Jahufer, M. Z. Z.; Barrett, B. A.; Griffiths, A. G.; Hofmann, R. W.
- Abstract
The use of molecular tools, coupled with improvements in paternity assignment computation, offer breeders a cost-effective and readily implementable breeding tool to improve the rate of genetic gain in forages. Molecular marker paternity assays may improve genetic gain in white clover (Trifolium repens L.) half-sib family breeding programs by utilizing a larger coeffi- cient of additive genetic variation. To evaluate the potential to improve breeding outcomes by use of a molecular marker paternity assay in allotetraploid white clover (2n = 4x = 32), seven single-locus, homoeologue-specific simple sequence repeat markers were genotyped and analyzed on 1277 half-sib progeny and their 20 parents from a polycross pollinated by bumble bees (Bombus sp.). Paternity was assigned to 88.9% of progeny using a likelihood algorithm implemented in Cervus. Estimates of additive genetic variances from multisite, space-planted field experiments established in perennial ryegrass (Lolium perenne L.) were used to predict selection responses. Joint maternal and molecular-marker-assayed paternal half-sib family selection offered improved theoretical selection gains relative to conventional maternal half-sib family selection for herbage yield and trifoliate center leaflet width. The estimated selection responses based on empirical parameters demonstrated the theoretical wisdom of incorporating molecular-marker-assayed paternity in white clover breeding programs as an effective tool to improve selection responses per cycle of selection.
- Subjects
WHITE clover yields; FORAGE plants; NUCLEIC acid isolation methods
- Publication
Crop Science, 2018, p617
- ISSN
0011-183X
- Publication type
Article
- DOI
10.2135/cropsci2017.06.0390