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- Title
Stereoselective antibodies to free α-hydroxy acids.
- Authors
Zeleke, Tigabu K.; Zeleke, Jessica M.; Hofstetter, Heike; Hofstetter, Oliver
- Abstract
This work describes antibodies exhibiting high stereoselectivity and class-specificity towards the enantiomers of free α-hydroxy acids. Since the antibodies interact primarily with the carboxyl-hydroxyl-hydrogen triad about the stereogenic center, they are useful for enantiomer analysis of a variety of structurally different α-hydroxy acids including aromatic and aliphatic compounds, e.g. lactic acid. The utility of such antibodies for enantiomer separation in chromatography was demonstrated. Comparative studies of these and previously described anti-α-amino acid antibodies revealed that both types of antibodies bind only to analytes that possess both the corresponding target structure and the correct configuration. Thus, substitution of an amino group for the α-hydroxyl group results in a complete loss of binding activity with the anti-α-hydroxy acid antibodies, while an α-amino group is essential for the interaction between analytes and anti-α-amino acid antibodies. Copyright © 2005 John Wiley & Sons, Ltd.
- Publication
Journal of Molecular Recognition, 2005, Vol 18, Issue 4, p334
- ISSN
0952-3499
- Publication type
Article
- DOI
10.1002/jmr.741