We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bioinspired engineering of fusogen and targeting moiety equipped nanovesicles.
- Authors
Wang, Lixue; Wang, Guosheng; Mao, Wenjun; Chen, Yundi; Rahman, Md. Mofizur; Zhu, Chuandong; Prisinzano, Peter M.; Kong, Bo; Wang, Jing; Lee, Luke P.; Wan, Yuan
- Abstract
Cell-derived small extracellular vesicles have been exploited as potent drug vehicles. However, significant challenges hamper their clinical translation, including inefficient cytosolic delivery, poor target-specificity, low yield, and inconsistency in production. Here, we report a bioinspired material, engineered fusogen and targeting moiety co-functionalized cell-derived nanovesicle (CNV) called eFT-CNV, as a drug vehicle. We show that universal eFT-CNVs can be produced by extrusion of genetically modified donor cells with high yield and consistency. We demonstrate that bioinspired eFT-CNVs can efficiently and selectively bind to targets and trigger membrane fusion, fulfilling endo-lysosomal escape and cytosolic drug delivery. We find that, compared to counterparts, eFT-CNVs significantly improve the treatment efficacy of drugs acting on cytosolic targets. We believe that our bioinspired eFT-CNVs will be promising and powerful tools for nanomedicine and precision medicine. Active targeting and cytosolic delivery of therapeutic payloads are challenging in small extracellular vesicle-based drug delivery systems. Here, the authors engineer fusogen and targeting moiety co-functionalized cell-derived nanovesicles, which can selectively bind to target cells and efficiently fulfill cytosolic delivery through membrane fusion.
- Subjects
DRUG delivery systems; DRUG carriers; MOIETIES (Chemistry); MEMBRANE fusion; NANOMEDICINE; EXTRACELLULAR vesicles; BIOLOGICALLY inspired computing
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-39181-2