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- Title
A rapid and sensitive enzymatic assay for 2,3-butanediol.
- Authors
Lee, Gyu Bi; Kim, Yun Jae; Lim, Jae Kyu; Kim, Tae Wan; Kang, Sung Gyun; Lee, Jung-Hyun; Lee, Hyun Sook
- Abstract
In this study, we developed a rapid and sensitive enzymatic assay for 2,3-butanediol (2,3-BDO) detection. The concentration of 2,3-BDO was determined by measuring the reduction of NADP+ using Clostridium ljungdahlii 2,3-butanediol dehydrogenase (CL-Bdh). The enzymatic assay could detect as low as 0.01 mM of 2,3-BDO, while the high-performance liquid chromatography (HPLC) method required a much higher concentration than 0.15 mM. Gratifyingly, the developed method was 15 times more sensitive than the HPLC method. When the enzymatic assay was applied to high-throughput screening, the enzymatic assay detected 14 positive samples out of 23 tested, as compared to 8 by the HPLC method. These results suggest that the enzymatic assay is an effective screening method for the detection of 2,3-BDO-producing microbes in a microtiter plate-based format.
- Subjects
LIQUID chromatography; CLOSTRIDIUM
- Publication
3 Biotech, 2019, Vol 9, Issue 5, pN.PAG
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-019-1705-9