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- Title
Change of Nurr1 expression in mouse hippocampal CA3 region following excitotoxic neuronal damage.
- Authors
Choong-Hyun Lee
- Abstract
Objective(s): Nuclear receptor-related protein 1 (Nurr1), one of immediate-early genes, is a member of orphan nuclear receptor family. The aim of this study was to investigate the time-dependent change of Nurr1 protein expression in the mouse hippocampal CA3 region following kainic acid (KA)-induced excitotoxic neuronal damage. Materials and Methods: Male ICR mice were used as experimental animals, and 30 mg/kg KA was administered intraperitoneally. To confirm the KA-induced neuronal damage in the hippocampal CA3 region, Fluoro-Jade B histofluorescence staining was performed. In addition, the time-dependent change of Nurr1 protein expression was also examined using immunohistochemistry and western blot analysis. Results: Marked neuronal damage was observed in the hippocampal CA3 region at 24 hr after KA injection. In addition, both Nurr1 immunoreactivity and protein level were significantly increased at 6 hr and 12 hr after KA injection, and then decreased at 24 hr after KA injection. Conclusion: This result indicates that KA-induced alteration of Nurr1 protein expression may be associated with the neuronal degeneration in the hippocampal CA3 region after KA injection.
- Subjects
WESTERN immunoblotting; KAINIC acid; PROTEIN expression; NUCLEAR proteins; NUCLEAR families
- Publication
Iranian Journal of Basic Medical Sciences, 2020, Vol 23, Issue 2, p202
- ISSN
2008-3866
- Publication type
Article
- DOI
10.22038/IJBMS.2019.38712.9177