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- Title
The bacterial replication origin BUS promotes nucleobase capture.
- Authors
Pelliciari, Simone; Bodet-Lefèvre, Salomé; Fenyk, Stepan; Stevens, Daniel; Winterhalter, Charles; Schramm, Frederic D.; Pintar, Sara; Burnham, Daniel R.; Merces, George; Richardson, Tomas T.; Tashiro, Yumiko; Hubbard, Julia; Yardimci, Hasan; Ilangovan, Aravindan; Murray, Heath
- Abstract
Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at the chromosome origin (oriC) that engages both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to promote DNA duplex opening. However, the mechanism of DnaA specifically opening a replication origin was unknown. Here we show that Bacillus subtilis DnaAATP assembles into a continuous oligomer at the site of DNA melting, extending from a dsDNA anchor to engage a single DNA strand. Within this complex, two nucleobases of each ssDNA binding motif (DnaA-trio) are captured within a dinucleotide binding pocket created by adjacent DnaA proteins. These results provide a molecular basis for DnaA specifically engaging the conserved sequence elements within the bacterial chromosome origin basal unwinding system (BUS). Here, the authors show that a DnaA:origin complex promotes specific nucleobase capture from a single DNA strand. It is proposed that this mechanism may play a key role stimulating opening of bacterial chromosome origins.
- Subjects
CHROMOSOME replication; DNA denaturation; BACTERIAL chromosomes; SINGLE-stranded DNA; DNA replication; BASE pairs
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-43823-w