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- Title
Changes in gingival crevicular fluid matrix metalloproteinase-8 levels during periodontal treatment and maintenance.
- Authors
Kinane, D. F.; Darby, I. B.; Said, S.; Luoto, H.; Sorsa, T.; Tikanoja, S.; Mäntylä, P.
- Abstract
Objectives: The aim of this study was to investigate the effect of scaling and root planing (SRP) and the maintenance phase of treatment on the gingival crevicular fluid (GCF) matrix metalloproteinase-8 (MMP-8) levels. Materials and methods: Clinical measurements and GCF samples were taken from four sites in 20 adult periodontitis patients before and after SRP and during a 3-month maintenance phase of treatment. MMP-8 levels were measured from GCF samples by time-resolved immunofluorometric assay (IFMA) with monoclonal antibodies. Results: SRP improved the clinical indices as would be predicted, 6.1 mm (SD = 1.4) at baseline compared with 4.3 mm (SD = 1.6) post-treatment (P < 0.001). Attachment level (AL) reduced but not significantly between these two visits 13.4 mm (SD = 2.4) compared with 12.8 mm (SD = 2.4) (P < 0.08) post therapy. GCF MMP-8 levels reduced after initial treatment from 33.8 μg/30 s sample to 23.5 μg/30 s, which just failed to reach statistical significance (P = 0.07). However, when MMP-8 levels were expressed as a concentration, the differences following initial therapy were significant (54.1 ng/μL at baseline compared with 34.2 ng/μL post treatment; P < 0.005). The difference, however, between the baseline MMP-8 levels (33.8 ng/30 s) and the final visit (16 ng/30 s) following maintenance was markedly significant (P < 0.001) for both absolute amounts and on a concentration basis. Conclusion: In conclusion, clinical improvement following SRP was associated with significant reductions in MMP-8 levels. The GCF concentration of MMP-8 decreased after initial therapy but reduced even more dramatically (approximately 50%) following a 3-month period of maintenance (P < 0.001).
- Subjects
GINGIVAL fluid; METALLOPROTEINS; IMMUNOGLOBULINS; THERAPEUTICS
- Publication
Journal of Periodontal Research, 2003, Vol 38, Issue 4, p400
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1034/j.1600-0765.2003.00663.x