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- Title
Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria.
- Authors
Karberg, Michael; Guo, Huatao; Zhong, Jin; Coon, Robert; Perutka, Jiri; Lambowitz, Alan M.
- Abstract
Mobile group II introns can be retargeted to insert into virtually any desired DNA target. Here we show that retargeted group II introns can be used for highly specific chromosomal gene disruption in Escherichia coil and other bacteria at frequencies of 0.1-22%. Furthermore, the introns can be used to introduce targeted chromosomal breaks, which can be repaired by transformation with a homologous DNA fragment, enabling the introduction of point mutations. Because of their wide host range, mobile group II introns should be useful for genetic engineering and functional genomics in a wide variety of bacteria.
- Subjects
INTRONS; BACTERIAL genetics; GENE targeting
- Publication
Nature Biotechnology, 2001, Vol 19, Issue 12, p1162
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt1201-1162