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- Title
SENSITIVITY AND SPECIFICITY OF MULTIPLEX PCR ASSAY FOR DETECTION OF YERSINIA ENTEROCOLITICA IN PIGS AND FOODS OF PORCINE ORIGIN.
- Authors
Boral, Rupa; Rathore, R. S.; Mishra, A. K.; Kumar, Ashok
- Abstract
Yersinia enterocolitica, an important food and water-borne gastrointestinal agent is regarded an emerging pathogen world wide. Animals that recover frequently become asymptomatic carriers of the disease. Most human illness is caused by Y. enterocolitica which causes a variety of symptoms depending on the age of the person infected often in young children. In the present study, a polymerase chain reaction (PCR) based assay was used for rapid detection of Y. enterocolitica targeting 16S rRNA, ail and yst genes in pig faeces, tongue, tonsils and ground pork samples. The primers were found to be highly specific for Y. enterocolitica and did not yielded any amplification with other Gram positive and Gram negative bacteria. The minimum detection level was found up to 10³ cells/ml for all the genes targeted in present assay. Prevalence rates were 5.83% in pig faeces; 6.67% in pig tongues; 18.3% in pig tonsils; 6.67% in pork, respectively. Overall prevalence rate was reported 8.34% with highest prevalence found in pig tonsils and lowest in pig faeces. PCR technique was found to be more efficient with 97.5% sensitivity than the cultural methods with only 90% sensitivity. Comparison studies revealed that the PCR assay was more rapid, specific, sensitive and efficient over conventional cultural and biochemical detection methods. To conclude, we could say that the PCR based detection is not only a sensitive but also a rapid and reliable method for detection of Y. enterocolitica in different sample matrices as clearly demonstrated by the results in present study.
- Subjects
YERSINIA enterocolitica; GRAM-negative bacteria; PORK; SWINE; POLYMERASE chain reaction; DISEASE vectors
- Publication
Veterinary Practitioner, 2019, Vol 20, Issue 2, p153
- ISSN
0972-4036
- Publication type
Article