We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice.
- Authors
Yukako Tani; Yosuke Isobe; Yuki Imoto; Eri Segi-Nishida; Yukihiko Sugimoto; Hiroyuki Arai; Makoto Arita
- Abstract
Resolution of inflammation is critical to restoration of tissue function after an inflammatory response. We previously demonstrated that 12/15-lioxygenase (12/15-LOX)-expressing eosinophils contribute to this process in murine zymosan-induced peritonitis. In this study, eosinophils promoted resolution by regulating expression of macrophage CXCL13. Microarray analysis revealed that eosinophils significantly increased (~ 3-fold) the expression of macrophage CXCL13 by a 12/15-LOX-dependent mechanism. CXCL13 depletion caused a resolution defect, with the reduced appearance of phagocytes carrying engulfed zymosan in the draining lymph nodes. Inflamed lymph node hypertrophy, a critical feature of the resolution process, was reduced by ~60% in eosinophil-deficient mice, and adoptive transfer of eosinophils or administration of CXCL13 corrected this defect. Administration of the 12/15-LOX-derived mediator lipoxin A4 (LXA4) increased the expression of CXCL13 and restored the defect of lymph node hypertrophy in eosinophil-deficient mice. These results demonstrate that eosinophils control the resolution of inflammation and draining lymph node hypertrophy through proresolving lipid mediators and the CXCL13 pathway in mice.
- Subjects
EOSINOPHILS; GRANULOCYTES; LYMPH nodes; LIPOXYGENASES; OXYGENASES
- Publication
FASEB Journal, 2014, Vol 28, Issue 9, p4036
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.14-251132