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- Title
Arrestin2.
- Authors
Gurevich, Eugenia V.; Benovic, Jeffrey L.; Gurevich, Vsevolod V.
- Abstract
Arrestins and G protein-coupled receptor kinases (GRKs) are key players in homologous desensitization of G protein-coupled receptors. Two non-visual arrestins, arrestin2 and 3, and five GRKs (GRK2, 3, 4, 5 and 6) are involved in desensitization of many receptors. Here, we demonstrate a steady increase in arrestin2 expression during prenatal development. The density of arrestin2 mRNA is higher in differentiated areas as compared with proliferative zones, whereas arrestin3 mRNA shows the opposite distribution. At embryonic day 14, concentrations of arrestin proteins are similar (32–34 nm). Later in development, arrestin2 expression rises, leading to a fourfold excess of arrestin2 over arrestin3 at birth (48 vs. 11 ng/mg protein or 102 vs. 25 nm). Among GRKs, only GRK5 increased with embryonic age from 124 nmat E14 to 359 nmat birth. Similarly,in vitrodifferentiation of cultured precursor cells, neurospheres, leads to a significant up-regulation of arrestin2 resulting in> 20-fold excess of arrestin2 (160 vs. 7 nm). GRK5 is the only subtype increased with neurosphere differentiation, although the change is only about twofold. The data demonstrate selective increases in the expression of arrestin2 associated with neural development and suggest specific yet unappreciated roles for arrestin2 in neural differentiation.
- Subjects
G proteins; MEMBRANE proteins; NEURON development; NEUROTROPHIC functions; DEVELOPMENTAL neurobiology; NEUROCHEMISTRY; NEUROSCIENCES; BIOCHEMISTRY
- Publication
Journal of Neurochemistry, 2004, Vol 91, Issue 6, p1404
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2004.02830.x