We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Assessment of liver ischemia reperfusion injury in mice using hepatic T<sub>2</sub> mapping: Comparison with histopathology.
- Authors
Hueper, Katja; Lang, Hannah; Hartleben, Björn; Gutberlet, Marcel; Derlin, Thorsten; Getzin, Tobias; Chen, Rongjun; Abou‐Rebyeh, Hassan; Lehner, Frank; Meier, Martin; Haller, Hermann; Wacker, Frank; Rong, Song; Gueler, Faikah; Abou-Rebyeh, Hassan
- Abstract
<bold>Background: </bold>Liver ischemia reperfusion injury (IRI) occurs during liver surgery or transplantation resulting in an inflammatory response, tissue damage, and functional impairment of the organ.<bold>Purpose: </bold>To assess the feasibility of T2 mapping for noninvasive quantification of liver edema after partial liver IRI in mice.<bold>Study Type: </bold>Prospective, experimental study.<bold>Animal Model: </bold>Partial liver IRI was induced in C57BL/6-mice by transient clamping of the left lateral and median liver lobes for 35 (n = 8), 45 (n = 6), 60 (n = 17), or 90 minutes (n = 5). For comparison, healthy C57BL/6-mice were examined as controls (n = 9).<bold>Field Strength/sequence: </bold>Functional liver MRI was performed on a 7T scanner using a respiratory-triggered multiecho spin-echo sequence.<bold>Assessment: </bold>Healthy control mice and mice with partial liver IRI on day 1 after surgery, and additionally on day 7 in a subgroup with 60 minutes IRI (n = 8) were examined. Maps of T2 relaxation time of liver tissue were used to assess distribution, severity of tissue edema (mean T2 time), and the percentage of edematous liver tissue.<bold>Statistical Test: </bold>One-way analysis of variance (ANOVA) with Tukey's honest significant difference (HSD), paired t-tests, Pearson's test for correlation of MRI parameters with levels of liver enzymes, and histopathology, receiver operating characteristic (ROC) analysis.<bold>Results: </bold>Significant tissue edema induced by liver IRI as compared to the control group was detected by increased mean T2 times in groups with 60 minutes (P < 0.001) and 90 minutes IRI (P < 0.001). The percentage of edematous liver tissue significantly increased with longer ischemia times (controls 3.4 ± 0.4%, 35 minutes 5.3 ± 0.6%, 45 minutes 23.3 ± 7.6%, 60 minutes 39.7 ± 3.6%, 90 minutes 51.3 ± 4.5%). Mean T2 times and the percentage of edematous liver tissue significantly correlated with elevation of liver enzymes (P < 0.001), histological evidence of liver injury (r = 0.80 and r = 0.82, P < 0.001), and neutrophil infiltration (r = 0.70 and r = 0.74, P < 0.001). In the subgroup with follow-up, the severity (P < 0.01) and extent of liver edema decreased significantly over time (P < 0.01).<bold>Data Conclusion: </bold>T2 mapping allows quantification and follow-up of liver injury in mice.<bold>Level Of Evidence: </bold>1 Technical Efficacy: Stage 2 J. Magn. Reson. Imaging 2018;48:1586-1594.
- Publication
Journal of Magnetic Resonance Imaging, 2018, Vol 48, Issue 6, p1586
- ISSN
1053-1807
- Publication type
journal article
- DOI
10.1002/jmri.26057