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- Title
Biophysical, Biochemical, and Molecular Docking Investigations of Anti-Glycating, Antioxidant, and Protein Structural Stability Potential of Garlic.
- Authors
Khan, Mohd W. A.; Otaibi, Ahmed A.; Alsukaibi, Abdulmohsen K. D.; Alshammari, Eida M.; Al-Zahrani, Salma A.; Sherwani, Subuhi; Khan, Wahid A.; Saha, Ritika; Verma, Smita R.; Ahmed, Nessar
- Abstract
Garlic has been reported to inhibit protein glycation, a process that underlies several disease processes, including chronic complications of diabetes mellitus. Biophysical, biochemical, and molecular docking investigations were conducted to assess anti-glycating, antioxidant, and protein structural protection activities of garlic. Results from spectral (UV and fluorescence) and circular dichroism (CD) analysis helped ascertain protein conformation and secondary structure protection against glycation to a significant extent. Further, garlic showed heat-induced protein denaturation inhibition activity (52.17%). It also inhibited glycation, advanced glycation end products (AGEs) formation as well as lent human serum albumin (HSA) protein structural stability, as revealed by reduction in browning intensity (65.23%), decrease in protein aggregation index (67.77%), and overall reduction in cross amyloid structure formation (33.26%) compared with positive controls (100%). The significant antioxidant nature of garlic was revealed by FRAP assay (58.23%) and DPPH assay (66.18%). Using molecular docking analysis, some of the important garlic metabolites were investigated for their interactions with the HSA molecule. Molecular docking analysis showed quercetin, a phenolic compound present in garlic, appears to be the most promising inhibitor of glucose interaction with the HSA molecule. Our findings show that garlic can prevent oxidative stress and glycation-induced biomolecular damage and that it can potentially be used in the treatment of several health conditions, including diabetes and other inflammatory diseases.
- Subjects
ADVANCED glycation end-products; MOLECULAR docking; PROTEIN stability; GARLIC; DENATURATION of proteins; PROTEIN conformation; DIABETES complications; SERUM albumin
- Publication
Molecules, 2022, Vol 27, Issue 6, p1868
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27061868