We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Identification of Anti-Inflammatory Compounds from Hawaiian Noni (Morinda citrifolia L.) Fruit Juice.
- Authors
Lee, Dahae; Yu, Jae Sik; Huang, Peng; Qader, Mallique; Manavalan, Arulmani; Wu, Xiaohua; Kim, Jin-Chul; Pang, Changhyun; Cao, Shugeng; Kang, Ki Sung; Kim, Ki Hyun; Juszczak, Lesław
- Abstract
Noni (Morinda citrifolia L.) fruit juice has been used in Polynesia as a traditional folk medicine and is very popular worldwide as a functional food supplement. In this study, compounds present in Hawaiian Noni fruit juice, with anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells were identified. Five compounds were isolated using a bioassay-driven technique and phytochemical analysis of noni fruit juice: asperulosidic acid (1), rutin (2), nonioside A (3), (2E,4E,7Z)-deca-2,4,7-trienoate-2-O-β-d-glucopyranosyl-β-d-glucopyranoside (4), and tricetin (5). The structures of these five compounds were determined via NMR spectroscopy and LC/MS. In an anti-inflammatory assay, compounds 1–5 inhibited the production of nitric oxide (NO), which is a proinflammatory mediator, in LPS-stimulated macrophages. Moreover, the mechanisms underlying the anti-inflammatory effects of compounds 1–5 were investigated. Parallel to the inhibition of NO production, treatment with compounds 1–5 downregulated the expression of IKKα/β, I-κBα, and NF-κB p65 in LPS-stimulated macrophages. Furthermore, treatment with compounds 1–5 downregulated the expression of nitric oxide synthase and cyclooxygenase-2. Thus, these data demonstrated that compounds 1–5 present in noni fruit juice, exhibited potential anti-inflammatory activity; these active compounds may contribute preventively and therapeutically against inflammatory diseases.
- Subjects
POLYNESIA; MORINDA citrifolia; FRUIT juices; NUCLEAR magnetic resonance spectroscopy; DIETARY supplements; CYCLOOXYGENASE 2
- Publication
Molecules, 2020, Vol 25, Issue 21, p4968
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules25214968