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- Title
Solution Properties and in Vitro Anti-Tumor Activities of Polysaccharides from Longan Pulp.
- Authors
Yang Yi; Fei Huang; Ming-Wei Zhang; Rui-Fen Zhang; Yuan-Yuan Deng; Zhen-Cheng Wei; Jing-Ren He
- Abstract
The solution properties of four fractions (LPI-IV) from crude longan pulp polysaccharides (LP3) were analyzed by size-exclusion chromatography combined with laser light scattering, viscometry, complex formation with Congo red, and atomic force microscopy. Their radii of gyration (<S2>z1/2) were 43.3, 62.6, 43.2 and 77.3 nm, exponents of <S2>z1/2 = k Mwv were 0.04, 0.50, 0.52 and 0.02, and intrinsic viscosities ([η]) were 9.945, 25.38, 308.2 and 452.1 mL/g, respectively. Moreover, the dependence of [η] on Mw was established to be [η] = 5.3 × 10-2Mw0.61 (mL/g). LPI had both a sphere-like conformation and a triple-helix structure, and LPII-IV existed as flexible chains. LP3, LPI, LPII and LPIII all exhibited direct inhibitory effects on A549, HeLa and HepG2 cells in a positive dose-dependent manner in the range of 50-400 μg/mL. The activities of LPIII, especially the inhibition of HepG2 cell proliferation, were stronger than those of others, which may be partly related to its flexible conformation. The present results support the cancer therapeutic potential of longan polysaccharides.
- Subjects
ANTINEOPLASTIC agents; POLYSACCHARIDES; CELL proliferation; TRIPLE-helix-forming oligonucleotides; GANODERMA lucidum; RANDOM coil (Polymers)
- Publication
Molecules, 2013, Vol 18, Issue 9, p11601
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules180911601