We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Sec62/Ki67 dual staining in cervical cytology specimens: a new marker for high-grade dysplasia.
- Authors
Takacs, Ferenc Zoltan; Radosa, Julia Caroline; Bohle, Rainer Maria; Bochen, Florian; Juhasz-Böss, Ingolf; Solomayer, Erich-Franz; Schick, Bernard; Linxweiler, Maximilian
- Abstract
<bold>Purpose: </bold>In the previous studies, we demonstrated that Sec62 is essential for tumor cell migration, epithelial-to-mesenchymal transition, and intracellular stress tolerance. An increase in Sec62 expression correlated with an increase in cervical dysplasia severity in liquid-based cytology specimens. Ki67 is an established proliferation marker. Thus, in this study, we examined a method of Sec62/Ki67 dual staining for the detection of high-grade dysplasia and cancer in cervical liquid-based cytology specimens.<bold>Methods: </bold>Sec62/Ki67 dual staining was performed on 100 cervical liquid-based cytology specimens. The staining results were correlated with cytological, immunocytological (p16/Ki67), colposcopic, and histological findings.<bold>Results: </bold>All 56 (n = 56, 100%) cases of cervical intraepithelial neoplasia grade 3 and cervical cancer (CIN3+ lesions) were positive for Sec62/Ki67 staining, while low-grade lesions and normal cells were negative. Sec62/Ki67 staining was highly sensitive and specific for the detection of CIN2+ and CIN3+ lesions (94.37%; 100% and 100%; 84.09%, respectively).<bold>Conclusions: </bold>Sec62/Ki67 dual-staining immunocytochemistry is a promising cytological tool for interpreting high-grade squamous lesions in cytological specimens and for assessing the risk of progression to cancer.
- Subjects
CERVICAL intraepithelial neoplasia; CYTOLOGY; CELL migration; CERVICAL cancer; CYTODIAGNOSIS; IMMUNOHISTOCHEMISTRY; PAP test; TUMOR markers; CERVIX uteri tumors; MEMBRANE transport proteins
- Publication
Archives of Gynecology & Obstetrics, 2019, Vol 299, Issue 2, p481
- ISSN
0932-0067
- Publication type
journal article
- DOI
10.1007/s00404-018-4981-4