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- Title
Complete genome sequence of a novel negevirus isolated from Culex tritaeniorhynchus in China.
- Authors
Wang, Yuanyuan; Guo, Xiaofang; Peng, Honghong; Lu, Yunlan; Zeng, Xucan; Dai, Ke; Zuo, Shuqing; Zhou, Hongning; Zhang, Jiusong; Tong, Yigang
- Abstract
A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus.
- Subjects
GENOMES; NUCLEOTIDE sequence; CULEX; MOSQUITOES; PHYLOGENY
- Publication
Archives of Virology, 2019, Vol 164, Issue 3, p907
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-018-04133-5