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- Title
Metasequencing of V3-V4 Variable Regions of 16S rRNA Gene in Opportunistic Microbiota and Gut Biocenosis in Obese Adolescents.
- Authors
Grigorova, E. V.; Belkova, N. L.; Nemchenko, U. M.; Klimenko, E. S.; Pogodina, A. V.; Romanitsa, A. I.; Novikova, E. A.; Rychkova, L. V.
- Abstract
Opportunistic microorganisms in the gut biocenosis were studied in adolescents with normal body weight and obesity (patients consulted at the Clinical Department of Research Center of Family Health and Human Reproduction Problems). The biological material was studied by standard bacteriological methods, representatives of Enterobacteriaceae family were also characterized using metagenomic sequencing of V3-V4 variable regions of 16S gene rRNA. Gut microbiota of obese adolescents was unbalanced and was characterized by low levels of bifido- and lactoflora representatives, a spectrum of E. coli associations, and high prevalence of opportunistic microorganisms and their associations. Representatives of Enterobacteriaceae family were most often found in the gut microbiota of obese adolescents.
- Subjects
GUT microbiome; TEENAGERS; OVERWEIGHT children; OBESITY; HUMAN reproduction; RESEARCH departments
- Publication
Bulletin of Experimental Biology & Medicine, 2021, Vol 170, Issue 3, p321
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-021-05060-3