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- Title
Hepatitis B Virus Genetic Typing Using Mass-Spectrometry.
- Authors
M. Malakhova; E. Ilina; V. Govorun; S. Shutko; K. Dudina; O. Znoyko; E. Klimova; N. Iushchuk
- Abstract
Mini-sequencing with subsequent result registration using MALDI-ToF mass-spectrometry was employed for hepatitis B virus genetic typing in Russian population. This approach was employed for hepatitis B virus genetic typing in HBsAg-positive patients with chronic hepatitis B, hepatitis of combined etiology and hepatic cirrhosis and allowed to show the prevalence of D genotype (83.3%) in all groups of patients. Other hepatitis B virus genotypes: genotype A (5.9%), genotype C (3.6%), and mixed infection with D and C (7.2%) were also found in patients with chronic hepatitis B and hepatic cirrhosis. All genotypes were found in patients with chronic hepatitis B and hepatic cirrhosis. Chronic hepatitis of combined etiology was noted only in patients with genotype D. Possibility of detection of mixed infection with hepatitis B viruses of various genotypes is a distinct advantage of mini-sequencing approach over direct nucleotide sequence evaluation for hepatitis B virus genetic typing.
- Subjects
HEPATITIS B virus; VIRAL genetics; MASS spectrometry; NUCLEOTIDE sequence; MATRIX-assisted laser desorption-ionization; RUSSIANS; POPULATION health; HEPATITIS B; PATIENTS; DISEASES
- Publication
Bulletin of Experimental Biology & Medicine, 2009, Vol 147, Issue 2, p220
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-009-0479-1