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- Title
Morphophysiology of Ananas comosus during in vitro photomixotrophic growth and ex vitro acclimatization.
- Authors
de Paula Alves, Juliana; Pinheiro, Marcos Vinícius Marques; Corrêa, Thais Roseli; Alves, Givago Lopes; dos Santos Marinho, Tácila Rayene; Batista, Diego Silva; de Assis Figueiredo, Fábio Afonso Mazzei Moura; de Oliveira Reis, Fabrício; Ferraz, Tiago Massi; Campostrini, Eliemar
- Abstract
Plants in conventional in vitro culture have a mixotrophic-type metabolism and they can present morphophysiological alterations that may prevent adequate photosynthetic capacity. For this, the objective was to verify whether photomixotrophic conditions could optimize photosynthesis, growth, and development of Ananas comosus under in vitro and ex vitro conditions. The experiment was carried out using a completely randomized design in a 2 × 3 factorial arrangement of treatments evaluating types of sealing (flasks with sealed lids or with permeable membranes) and sucrose concentrations (10, 20, and 30 g L−1) with five replications and the experimental unit consisted of one flask containing three plants each. After 90 d of in vitro culture, the following parameters were assessed: shoot and longest root length; number of leaves; leaf area; specific leaf mass; shoot and root fresh and dry mass and the ratio of these parameters; total dry mass; photoassimilate partitioning between roots and shoots; maximum photosystem II quantum yield (Fv/Fm); photosynthetic index (PI); photosystem II active reaction center density per unit of photons absorbed (RC/ABS); net CO2 assimilation rate at photosynthetically active radiation (PAR) of 100 μmol m-2 s-1 (A100); SPAD index; photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, chlorophyll a/b ratio and total chlorophyll (a+b)/carotenoids) ratio; and stomata index. Five plants from each treatment were also assessed at 5, 10, and 15 d after acclimatization for Fv/Fm, A at PAR of 500 μmol m-2 s-1 (A500); PI, and SPAD. The use of porous membranes associated with sucrose (10 g L−1) resulted in longer shoot lengths, higher leaf area, increased shoot length, higher photosynthetic capacity, chlorophyll contents, and carotenoids. Therefore, for photomixotrophic in vitro culture of A. comosus, the use of permeable membranes and 10 g L−1 sucrose benefited the plant growth and potentialized ex vitro acclimatization.
- Subjects
PINEAPPLE; ACCLIMATIZATION (Plants); ACCLIMATIZATION; PHOTOSYNTHETIC pigments; LEAF area; PHOTOSYSTEMS
- Publication
In Vitro Cellular & Developmental Biology Plant, 2023, Vol 59, Issue 1, p106
- ISSN
1054-5476
- Publication type
Article
- DOI
10.1007/s11627-022-10321-5