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- Title
A DNA-based method for investigating feeding by copepod nauplii.
- Authors
Craig, Carrie; Kimmerer, Wim J.; Cohen, C. Sarah
- Abstract
We developed an approach to recover prey DNA from gut contents of nauplii of a predatory copepod by blocking the detection of predator with ligase and a blocking oligonucleotide and amplifying prey DNA with universal primers. Adding 25 U of ligase improved predator clamping, while 100 and 200 U reduced efficiency. Field-collected nauplii consumed a broad range of prey. The method paves the way to investigations of the diets of small predators at all life stages.
- Subjects
COPEPODA; PREDATION; LIGASES; OLIGONUCLEOTIDES; DNA primers; DEVELOPMENTAL biology; FOOD
- Publication
Journal of Plankton Research, 2014, Vol 36, Issue 1, p271
- ISSN
0142-7873
- Publication type
Article
- DOI
10.1093/plankt/fbt104