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- Title
Effect of Feeding Vitamin D Supplementation on Calcium and Phosphorus Metabolism When Piglets Are Fed a DON Contaminated Diet.
- Authors
Sauvé, Béatrice; Létourneau-Montminy, Marie-Pierre; Guay, Frédéric
- Abstract
DON has been shown to induce anorexia, oxidative stress, and more recently, alterations in calcium (Ca) and phosphorus (P) metabolism. It was thus hypothesized that vitamin D3 or 25-OH-D3 can counteract the effects of DON in piglets. The objectives were to confirm the DON effect and to evaluate the impact of a vitamin D supplementation on growth performance and Ca and P metabolism on piglets receiving a DON contaminated feed. A total of 66 piglets received one of the 6 treatments in 2 x 3 factorial design: control treatments (CON) or DON unsupplemented or supplemented with VitD3 or 25-OH-D3. After 21 days, piglets were weighted and feed intake was measured. Bone mineral content of each piglet was measured with dualintensity X-ray absorptiometry at 21 days. Blood samples were taken to assess concentrations for VitD, P and Ca. Results showed that, DON reduced average daily feed intake and average daily gain (P < 0.001). DON increased BMC per kg live body weight (P < 0.001). Piglets fed DON diet had lower plasma concentrations of 25-OH-D3, 1,25-(OH)2-D3 and phosphate (P < 0.001). The sodium-dependent phosphate transporter 1 (SLC20A2; P < 0.050), sodium/calcium exchanger 1 (SLC8A1; P < 0.032) and Klotho (P < 0.014) gene expression was downregulated by DON contamination in intestinal mucosae. The calbindin d28K (CALB-1) (P < 0.042), SLC8A1 (P < 0.026), TRPV5 (P < 0.041), calbindin d9K (S100G) (P < 0.029) and Klotho (P < 0.013) gene expression was downregulated by DON contamination in the kidney. The 1α-hydroxylase gene expression tended to be downregulated by DON contamination in the kidney as well. The VitD3 and 25-OH-D3 supplementation upregulated vitamin D receptor (VDR, DON x VitD, P < 0.014), SLC8A1 (DON x VitD, P < 0.036) and Klotho (DON x VitD, P < 0.025) in intestinal mucosae, although those gene expressions were downregulated by DON contamination. Thus, DON induced an alteration of vitamin D-calcium-phosphorus metabolism by modifying Ca utilization from mechanisms still under investigation.
- Subjects
CALCIUM metabolism; PHOSPHORUS metabolism; DIETARY supplements; VITAMIN D; VITAMIN D receptors; PIGLETS
- Publication
Journal of Animal Science, 2021, Vol 99, p30
- ISSN
0021-8812
- Publication type
Article
- DOI
10.1093/jas/skab054.053