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- Title
Frequent and Durable Anti-HIV Envelope VIV2 IgG Responses Induced by HIV-1 DNA Priming and HIV-MVA Boosting in Healthy Tanzanian Volunteers.
- Authors
Joachim, Agricola; Msafiri, Frank; Onkar, Sayali; Munseri, Patricia; Aboud, Said; Lyamuya, Eligius F.; Bakari, Muhammad; Billings, Erik; Robb, Merlin L.; Wahren, Britta; Mhalu, Fred S.; Sandström, Eric; Rao, Mangala; Nilsson, Charlotta; Biberfeld, Gunnel
- Abstract
We evaluated antibody responses to the human immunodeficiency virus (HIV) envelope variable regions 1 and 2 (V1V2) in 29 vaccinees who had received three HIV-1 DNA immunizations and two HIV-modified vaccinia virus Ankara (MVA) boosts in the phase I/II HIVIS03 vaccine trial. Twenty vaccinees received a third HIV-MVA boost after three years in the HIVIS06 trial. IgG and IgG antibody subclasses to gp70V1V2 proteins of HIV-1 A244, CN54, Consensus C, and Case A2 were analysed using an enzyme-linked immunosorbent assay (ELISA). Cyclic V2 peptides of A244, Consensus C, and MN were used in a surface plasmon resonance (SPR) assay. Four weeks after the second HIV-MVA, anti-V1V2 IgG antibodies to A244 were detected in 97% of HIVIS03 vaccinees, in 75% three years later, and in 95% after the third HIV-MVA. Anti-CN54 V1V2 IgG was detectable in 48% four weeks after the second HIV-MVA. The SPR data supported the findings. The IgG response was predominantly IgG1. Four weeks after the second HIV-MVA, 85% of vaccinees had IgG1 antibodies to V1V2 A244, which persisted in 25% for three-years. IgG3 and IgG4 antibodies to V1V2 A244 were rare. In conclusion, the HIV-DNA/MVA vaccine regimen induced durable V1V2 IgG antibody responses in a high proportion of vaccinees.
- Subjects
ANKARA (Turkey); HIV antibodies; SURFACE plasmon resonance; ENZYME-linked immunosorbent assay; VACCINIA; CYCLIC peptides
- Publication
Vaccines, 2020, Vol 8, Issue 4, p681
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines8040681